Dysplastic Kidney

EVALUATION OF THE PATHOGENICITY PROPERTY OF Fusarium graminearum in a BALB / c

Kedar B. Karki and Gemerlyn G. Garcia 2

Summary

An experiment was conducted to assess the immunological property, pathogenicity and treatment of infection by Fusarium graminearum. Several groups of mice were randomly selected for the following groups: (PC, T1 and T2 were respectively the groups of mice received a 1:1, 1:100 and 1:100,000 dilution fungal T5 while T3, T4, and groups of mice that were, respectively, received the same concentration, but each was treated with diethylamine Acetarsol (Acetylarsan). A group of mice was included as a negative control (NC).

In vitro assays were used to examine the ability of F. graminearum to produce enzymes, which are considered important indicators of virulence. The results showed the pathogen's ability to produce collagenase and elastase. In addition, histopathological examination indicated the vascular congestion and mild liver triaditis. pulmonary congestion and lymphoid hyperplasia in the spleen were noted. Fungi were recovered from the liver, lungs, spleen and skin of the legs of certain animals. Similarly, increased spleen weights doubled in the second week (from 49 mg to 80 mg) and has progressed to the fourth week (125 mg), where it tapered off in the untreated group. similar increase in spleen weight was observed in the treated group (40 mg to 64 mg), but not as great as that in the untreated group (105 mg). Blood results showed a lymphocyte count rose from 1.83 to 3.356, number of monocytes that increased from 0.47 to 0.981 and neutrophils increased from 0.399 to 1.698 in the treated groups. the number of lymphocytes in the treated group increased from 1.8 to 3.64, the monocytes increased from 0.068 to 0.325 and neutrophils increased from 0.223 to 1.056. the high incidence of death was observed in animals that received no treatment (PC, T1 and T2), while the relatively low incidence of mortality have been exposed by groups that received diethylamine acetarsol (T3, T4 and T5).



1 A thesis by the author Masteral Lord submitted to the Institute of Graduate Studies, Central Luzon State University, graduate student of science city of Muaħoz, Nueva Ecija

2 Assistant Professor of Microbiology and Chairman of the Institute of Graduate Studies Faculty of Veterinary Medicine.

INTRODUCTION



There is insufficient information describing the pathogenicity of the fungus Fusarium in cattle in different parts of the globe. This fungus has been associated with the disease Deg Nala. It mainly affects cattle and buffaloes in India, Pakistan and Nepal.

The precise mechanisms underlying the symptoms of Deg Nala disease is not known. In this study, the investigative efforts had been focused on the pathogenicity, the ability to produce an immune response and efficacy of acetarsol diethylamine as effective therapeutic agent.

Problem Statement

Raising cattle and buffaloes in Pakistan, Nepal and India is a way to increase the financial resources of the villagers. The infections can be debilitating in nature can cause significant economic losses due to decreased production confounded by reduced growth rate, mortality and poor animal performance. An effort to improve livestock production in the village requires a proper control or treatment measures of disease. Experimental evaluation of immunological properties and treatment of infections F. graminearum must be considered.

Study Objectives

The overall objective of this study was to determine pathogenicity, and immunological characteristics.